Escherichia coli|364435 |BNCC

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Escherichia coli-BNCC
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  • Escherichia coli (Migula) Castellani and Chalmers-BNCC

Escherichia coli (Migula) Castellani and Chalmers

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  • number：364435
  • Form：
    The colony diameter is 1-2mm, circular, with neat edges, opaque, yellow on the front, light color, raised in the middle, smooth surface, bright surface, moist texture, easy to pick up, G - (red), Bacillus, purity: pure
Standard strain Quantitative strain DNA extraction
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Escherichia coli (Migula) Castellani and Chalmers
Subculture procedure ① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions 37 ℃; 18-24h; aerobic
Storage conditions 2-8 ℃
Safety level 1
morphology The colony diameter is 1-2mm, circular, with neat edges, opaque, yellow on the front, light color, raised in the middle, smooth surface, bright surface, moist texture, easy to pick up, G - (red), Bacillus, purity: pure
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