Histidine deficient Salmonella enterica TA97a|365086 |BNCC

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number：365086
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Verification method: Inoculate the strain into nutrient broth medium and culture on a shaker at 37 ℃ for 18-24 hours. The bacterial solution is significantly turbid. Then dip the inoculation ring into the bacterial solution and streak culture on the medium containing L-histidine and the medium without L-histidine, respectively. After 24 hours of cultivation at 37 ℃, observe. The strain grows well on the surface of the L-histidine containing medium Agar plate (left image), but is inhibited in the absence of L-histidine medium Agar plate, and does not grow or grows a few colonies (right image)

Standard strain Quantitative strain DNA extraction

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Salmonella enterica（his） TA97a

Subculture procedure	① Prepare one 50mL nutrient broth triangular bottle; ② Use sterile tweezers to remove one from the biosafety cabinet and place it in a triangular bottle; ③ Place the triangular flask on a shaking table at 37 ℃ for shaking cultivation, and achieve a viable bacterial count of (1-2) x10 ^ 9/mL.
Growth conditions	37 ℃; 18-24h; aerobic
Storage conditions	2-8 ℃
Safety level	2
morphology	Verification method: Inoculate the strain into nutrient broth medium and culture on a shaker at 37 ℃ for 18-24 hours. The bacterial solution is significantly turbid. Then dip the inoculation ring into the bacterial solution and streak culture on the medium containing L-histidine and the medium without L-histidine, respectively. After 24 hours of cultivation at 37 ℃, observe. The strain grows well on the surface of the L-histidine containing medium Agar plate (left image), but is inhibited in the absence of L-histidine medium Agar plate, and does not grow or grows a few colonies (right image)
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