Hericium erinaceus 99|340819 |BNCC

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Hericium erinaceus

Subculture procedure	① Prepare 1-2 plates as mentioned above; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the Agar plate and onto the surface of the agar; ⑤ Cultivate the flat Agar plate under the above cultivation conditions, and the strain can be used once it grows.
Growth conditions	25-28 ℃; 7-10 days; aerobic;
Storage conditions	-80 ℃
Safety level	1
morphology	Small filamentous fungi have obvious colonies on comprehensive PDA medium, with curved and white hyphae, sparse and flat colonies, spreading and growing towards the edge of the Agar plate, and do not produce spores
Sharing mode	Public welfare sharing

Description

1. Name: Hericium erinaceus

2. BNCC No.: 340819

3. Biosafety level: 4

Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

Growth Conditions

1. Comprehensive PDA: potato boiled juice 1000mL, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, glucose 20g, vitamin B1 10mg, agar 20g,pH natural. Potato boiling solution: 200g of potato, peeled, cut into pieces, boiled in distilled water for 30min, constant volume of filtrate to 1000mL for later use. 121 ℃,15min.

2. Atmosphere: aerobic

3, Temperature: 25-28 ℃

Notes:
1.Normal culturing time, 1-2 days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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