Herbaceous mycorrhizal fungi|144751 |BNCC

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Cladosporium herbarum

Subculture procedure	Small filamentous fungi have obvious colonies on comprehensive PDA medium, with initially gray white hyphae that are dense and vigorous, do not spread and grow, and later produce dark green spores
Growth conditions	15% skim milk
Storage conditions	Freeze dried pellet
Safety level	1
morphology	A small filamentous fungus, with distinct colonies on comprehensive PDA medium, initially exhibits grayish-white, dense, and vigorous hyphae that do not spread, later producing dark green spores.
Sharing mode	Public welfare sharing

Cladosporium herbarum

Storage conditions: 2~8 ℃

No. 144751

Product format： freeze dried, 200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 25-28℃, aerobic, integrated PDA, 5-7 days. Comprehensive PDA: Potato cooking liquid 1.0L, glucose 20.0g, KH2PO4 3.0g, MgSO4·7H2O 1.5g, trace amount of vitamin B1, agar 20.0g, pH 6.0±0.2. Sterilize at 121℃ for 15min. Potato cooking liquid: Weigh 200g of peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Recovery steps:

(1)Prepare 2 of above mentioned plates;

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions for cultivation

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability,in 5-7 days strain layer become obvious, colony is typical
colony morphology:	filamentous fungi, the colonies are obvious on the comprehensive PDA medium, and the hyphae are vigorously spreading and growing, The initial hyphae are gray and dense, and the later hyphae produce black spores
Conclusion:	good viability, no abnormal colony morphology, qualified

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