BeNa Culture Collection
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| Subculture procedure | ① Prepare 1-2 plates as mentioned above; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the Agar plate and onto the surface of the agar; ⑤ Cultivate the flat Agar plate under the above cultivation conditions, and the strain can be used once it grows. |
| Growth conditions | 28 ℃, 5-7 days, aerobic |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | A small filamentous fungus, with distinct colonies on comprehensive PDA medium. The mycelium is initially white and later turns pink. The colonies are fluffy and spreading, with a central point on the reverse side that is brownish-yellow, gradually fading to the edges. The aerial mycelium is also fluffy. |
| Sharing mode | Public welfare sharing |
Fusarium moniliforme
Storage conditions : 2~8 ℃
No.: 195387
Product format: agar slant in 14mm test tube
Validity period: 30 days
Biosafety level : 1, handle in ultra-clear table or safety cabinet
Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.
Growth conditions:25-28 ℃, aerobic, integrated PDA,5-7 days. Comprehensive PDA: potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Recovery steps:
(1)Prepare 1-2 pieces of PDA plates;
(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;
(3)Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2;
(4)Lay flat the small pieces to the center of the agar plate;
(5)Put the plates under the above culture conditions, and the strains can be used when they grow.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:
| item | test result |
| viability | good viability, in 5 days plate colony is obvious |
| colony morphology: | Small filamentous fungi have obvious colonies on comprehensive PDA culture medium. The hyphae are white at the initial stage and pink at the later stage. The colonies are fluffy and spread. The center of the reverse surface is scorched yellow, gradually shallower to the edge, and the air-generated hyphae are fluffy. |
| Conclusion: | good viability, no abnormal colony morphology, qualified |
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