Botrytis cinerea|368521 |BNCC

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Hypsizygus marmoreus

Culture medium	BNCC352233
Description	Potato glucose agar
Composition	Potato Infusion Powder:6.0g, Glucose:20.0g, Agar:20.0g, pH:5.6±0.2(25℃)
Growth conditions	21 ℃; 15-20 days; aerobic;
Subculture procedure	① Dissolve the frozen tube in a 25 ℃ water bath and shake quickly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each Agar plate contains approximately 200 μ L, and for anaerobic bacteria cultivation, the culture medium needs to be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Storage conditions	-80 ℃
Safety level	1
morphology	Small filamentous fungi grow well on PDA medium, producing white mycelium on the front, dense mycelium, spreading growth, and yellow on the back of the medium
Sharing mode	Public welfare sharing