Fusarium oxysporum var. anisopliae|336557 |BNCC

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Metarhizium anisopliae var. anisopliae

Subculture procedure	① Prepare 1-2 plates as mentioned above; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	28 ℃; 7-10 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Spread and grow, produce yellow green spores, irregular edges, opaque, yellow front, rough surface, dry texture
Sharing mode	Public welfare sharing

Metarhiziumanisopliaevar.anisopliae

Description

1. BNCC No.:336557

2. Biosafety level: 4

Storage conditions

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

Growth Conditions

1. comprehensive PDA: potato extract 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4.7H2O 1.5g, vitamin B1 trace, agar 15.0g,pH natural. [Note] Potato extract: 200g of peeled potato is taken, cut into small pieces, add 1.0L of water to boil for 20min, filter out potato pieces, and supplement the filtrate to 1.0L.

2. Atmosphere: aerobic

3. Temperature: 25-28 ℃

Notes:

1.Normal culturing time, 24-48 hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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