BeNa Culture Collection
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| Culture medium | BNCC352239 |
| Description | Nutrient Agar |
| Composition | Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃) |
| Growth conditions | 37°C; 18-24h; aerobic; |
| Subculture procedure | ① Prepare 1-2 plates as above; ② Open in a safety cabinet, cauterize the top with a spirit lamp, then quickly add sterile water to break it open; use forceps to crush it; ③ Pipette 0.5mL sterile water into the freeze-dried vial, dissolve the powder completely, then dispense 200μL/well onto plates, spreading evenly; ④ Incubate plates under the specified conditions; the strain is ready for use once growth appears. |
| Storage conditions | 2-8°C |
| Safety level | 2 |
| morphology | Colony diameter: 1-2 mm; circular, with neat edges; opaque, grayish-white upper surface; centrally convex; smooth, glossy surface; moist texture; easily liftable; non-pigmented; Gram-negative (red); bacillus; purity: pure |
| Sharing mode | Public welfare sharing |
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