Escherichia coli|374451 |BNCC

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Escherichia coli (Migula) Castellani and Chalmers

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	37°C; 18-24h; aerobic;
Subculture procedure	① Prepare 1-2 plates as above; ② Open in a safety cabinet, cauterize the top with a spirit lamp, then quickly add sterile water to break it open; use forceps to crush it; ③ Pipette 0.5mL sterile water into the freeze-dried vial, dissolve the powder completely, then dispense 200μL/well onto plates, spreading evenly; ④ Incubate plates under the specified conditions; the strain is ready for use once growth appears.
Storage conditions	2-8°C
Safety level	2
morphology	Colony diameter: 1-2 mm; circular, with neat edges; opaque, grayish-white upper surface; centrally convex; smooth, glossy surface; moist texture; easily liftable; non-pigmented; Gram-negative (red); bacillus; purity: pure
Sharing mode	Public welfare sharing