Nocardia trifasciata|373537 |BNCC

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Nocardioides sambongensis

Culture medium	BNCC352246
Description	R2A Agar
Composition	Peptone:0.5g, Casein Hydrolysate:0.5g, Yeast Extract:0.5g, Soluble Starch:0.5g, K₂HPO₄:0.3g, MgSO₄:0.024g, Sodium Pyruvate(C₃H₃NaO₃):0.3g, Agar:15.0g, Glucose:0.5g, pH:7.2±0.2(25℃)
Growth conditions	28°C; 48-72h; aerobic
Subculture procedure	① Prepare 1-2 plates as above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then rapidly add sterile water to rupture. Immediately crush with forceps; ③ Pipette 0.5mL sterile water into lyophilized tube. After complete dissolution, dispense 200μL/Agar plate and spread evenly; ④ Incubate plates under specified conditions until colonies emerge.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm; circular shape with neat margins; opaque; white upper surface with central elevation; smooth, glossy surface; viscous, moist texture; non-pigmented; Gram-positive (blue-violet); bacillus; purity: pure
Sharing mode	Public welfare sharing