Picky snail fungus|383991 |BNCC

Essential Information Related Products

Lachnospira eligens

Culture medium	BNCC384501
Description	Ruminococcus Medium Base with Cellobiose
Composition	Tryptone:5.0g, Yeast extract:2.0g, Glucose:3.0g, Cellobiose:2.0g, Resazurin:0.001g, Na₂CO₃:4.0g, C₃H₇NO₂S.HCl:0.5g, Mineral solution 1:K₂HPO₄:0.24g, Mineral solution 2:KH₂PO₄:0.24g, (NH₄)₂SO₄:0.8g, NaCl:0.48g, MgSO₄.7H₂O:0.1g, CaCl₂.7H₂O:0.064g, Volatile fatty acid solution:1mL
Growth conditions	37 ℃; 3-7 days; anaerobic
Subculture procedure	① Prepare 1 test tube of liquid culture medium (placed in anaerobic environment for 24 hours of deoxygenation in advance); ② Dissolve the Frozen vials in a 37 ℃ water bath and shake rapidly for dissolution; Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to an anaerobic glove box for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer the bacterial solution to a new liquid culture medium using a pipette or suction tube in an anaerobic environment; ④ Incubate the liquid test tube under the above cultivation conditions until the bacterial solution becomes turbid and ready for use.
Storage conditions	-80 ℃
Safety level	1
morphology	G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing