Clostridium perfringens|387047 |BNCC

BeNa Culture Collection

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Clostridium perfringens-BNCC
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Clostridium perfringens

  • Price： $ 372
  • number：BNCC387047
  • MPs：
    Colony diameter: 1-2 mm; circular, with neat margins; opaque, grayish-white upper surface; convex center; smooth, shiny surface; moist texture; easily lifted; G+ (blue-violet); bacilli; purity: pure
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Clostridium perfringens
Culture medium BNCC352241
Description Columbia blood agar plate
Composition Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions 37°C; 18-24h; anaerobic
Subculture procedure ① Prepare two plates as above (equilibrated in anaerobic conditions for 24h); ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then rapidly add sterile water to rupture; break open with forceps; ③ Pipette approximately 0.5 mL sterile water (pre-equilibrated in anaerobic conditions for 24 hours) into the lyophilized vial. Thoroughly dissolve the bacterial powder, then inoculate two plates with 200 μL each, spreading evenly; ④ Incubate plates under the aforementioned culture conditions.
Storage conditions 2-8°C
Safety level 2
morphology Colony diameter: 1-2 mm; circular, with neat margins; opaque, grayish-white upper surface; convex center; smooth, shiny surface; moist texture; easily lifted; G+ (blue-violet); bacilli; purity: pure
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