Beauveria bassiana|122059 |BNCC

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Beauveria bassiana (Bals.-Criv.) Vuill.

Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	28 ℃, 5-7 days, aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Small filamentous fungi have obvious colonies on comprehensive PDA medium, with white, powdery, dense and vigorous hyphae that spread and grow towards the edge of the Agar plate. The back of the medium is light yellow
Sharing mode	Public welfare sharing

Beauveria bassiana

Storage conditions : 2~8 ℃

No. 122059

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions : 28°C, aerobic, integrated PDA, 5-7 days. Comprehensive PDA: Potato cooking liquid 1.0L, glucose 20.0g, KH2PO4 3.0g, MgSO4 7H2O 1.5g, vitamin B1 trace, agar 20.0g, pH 6.0±0.2. Sterilize at 121℃ for 15min. Potato cooking liquid: Weigh 200g of peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Recovery steps:
(1)Prepare 1-2 of above mentioned plates;
(2)Open the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3)Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4)Put the plates under the above culture conditions, and the strains can be used when they grow.

recovery quality record: activation is carried out according to activation requirements, and the recording results are as follows:

item	test results
viability	good viability, in 5 days plate colonies are obvious
colony morphology:	small filamentous fungi, the colonies are obvious on the comprehensive PDA medium, the hyphae are white, powdery, dense and vigorous, spreading to the edge of the plate, and the back of the medium is light yellow.
Conclusion:	good viability, no abnormal colony morphology, qualified

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