BeNa Culture Collection
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| Subculture procedure | ① Prepare 1 tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to break it, followed by crushing it with forceps; ③ Pipette 0.5 mL liquid medium into the lyophilized tube, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2 mL bacterial suspension onto a Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid test tubes and plates under the specified conditions; the culture is ready for use once growth appears. |
| Growth conditions | 30°C, 18-24h, aerobic |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 1-2 mm; circular shape with neat margins; opaque; yellow upper surface; centrally raised; glossy surface; smooth texture; moist consistency; easily liftable; Gram-positive (blue-violet); bacilli |
| Sharing mode | Public welfare sharing |
Bacillus fusiformis
Storage conditions: 2~8 ℃
No.: 132602
Product format : freeze dried,200ul
Validity period: Freeze-dried tube for 24 months
Biosafety level: 1, handle in ultra-clear table or safety cabinet
Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.
Recovery/subculture:30°C, aerobic, nutrient agar/gravy medium (NA/NB). Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH7.0. 121 ℃,15min.
Recovery steps:
(1)Prepare a flask of NB liquid media or two NA agar plates.
(2)Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3)Draw 0.5mL of liquid medium into the freeze dried ampoule,make the strain pellet fully dissolved. transfer the liquid suspension to the liquid media, and culture in plate shaker at 30℃ (140r/min); or directly dispense 200ul of the liquid suspension into a NA agar plate evenly, then put the plates in incubator at 30℃ for 18-24 hours.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:
| item | test result |
| viability | good viability, in 20h NB bacterial fluid become turbid, NA plate colony is obvious |
| colony morphology: |
size: medium color: yellow shape: round edge: neat edge wet and dry: wet and smooth: smooth transparency: opaque uplift: low convex |
| Conclusion: | good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified |
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