Bacillus johnsonii|132605 |BNCC

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Bacillu gibsonii

Subculture procedure	① Prepare two plates as above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then rapidly add sterile water to rupture. Immediately crush with forceps; ③ Pipette 0.5 mL sterile water into lyophilized tube. Thoroughly dissolve powder, then dispense 200 μL onto each of 2 plates, spreading evenly; ④ Incubate plates under specified conditions until colonies emerge.
Growth conditions	30°C; 18-24h; aerobic
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm; circular shape with neat, opaque edges; yellow upper surface; central elevation; smooth, glossy surface; moist texture; easily liftable; G+ (blue-violet); bacilli; purity: pure
Sharing mode	Public welfare sharing

Bacillu gibsonii

Storage conditions: 2~8 ℃

No. 132605

Product format: freeze dried,200ul

Validity period: Freeze-dried tube for 24 months

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Recovery/subculture:30°C, aerobic, nutritious gravy medium (NB). Nutritional gravy medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, distilled water 1.0L, pH7.0. 121 ℃,15min.

Recovery steps:
(1) Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;
(2) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;
(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;
(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability:	good viability, in 20h,NB bacterial fluid is turbid; The bacterial liquid is marked with NA plate, and the colony is obvious
colony morphology:	size: medium ; Color: orange ; Shape: round ; ; Edge: neat edges/p> wet and dry: wetter ; Smooth: rougher ; Transparency: opaque ; Protuberance: flat
Conclusion	good viability, and the colony morphology is not abnormal, which is completely consistent with the above figure and is qualified.

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