BeNa Culture Collection
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| Culture medium | BNCC360795 |
| Description | Nutrient Blood Agar |
| Composition | Beef Extract:3.0g, Peptone:10.0g, NaCl:5.0g, Agar:15.0g, pH:7.0(25℃) |
| Growth conditions | 30°C, 18-24h, aerobic. |
| Subculture procedure | ① Prepare 1 test tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, flame the top with an alcohol lamp, then quickly add sterile water to break it; subsequently crush it with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried vial, dissolve thoroughly, then return to the liquid test tube and mix well; ④ Pipette 0.2mL of the bacterial suspension onto a Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the bacterial strain grows. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 0.5–1mm; circular, with neat edges; opaque, grayish-white on the upper surface; flat morphology; smooth surface; easily liftable; G+ (blue-violet); bacillus |
| Sharing mode | Public welfare sharing |
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