Staphylococcus aureus subspecies aureus|134242 |BNCC

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Staphylococcus aureus subsp. aureus

Subculture procedure	① Prepare 1 tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with a spirit lamp, then quickly add sterile water to shatter it; break into pieces with forceps; ③ Pipette 0.5 mL liquid medium into the lyophilized tube, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2 mL bacterial suspension onto plates, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid test tubes and plates under the specified conditions; the strain is ready for use once growth appears.
Growth conditions	37°C, 18-24h, aerobic
Storage conditions	2-8°C
Safety level	2
morphology	Colony diameter: 0.5–1 mm; circular, with neat edges; opaque; golden yellow on top; centrally convex; smooth surface; glossy appearance; moist texture; easily lifted; Gram-positive (blue-violet); cocci
Sharing mode	Public welfare sharing

Staphylococcus aureus subsp

Storage conditions : 2~8 ℃

No. : 134242

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 2, handle in biosafety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :37°C, aerobic, nutrient agar/gravy medium. Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH7.0. 121 ℃,15min.

Recovery steps:

(1)Prepare a flask of NB liquid media or two NA agar plates.

(2)Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid medium into the freeze dried ampoule,make the strain pellet fully dissolved. transfer the liquid suspension to the liquid media, and culture in plate shaker at 37℃ ; or directly dispense 200ul of the liquid suspension into a NA agar plate evenly, then put the plates in incubator at 37℃ for 18-24 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability:	good viability, in 20h NB bacterial fluid become turbid, NA plate colony obvious
Colony morphology: (pictured above)	size: medium shape: round edge: neat edge transparency: opaque color: light yellow uplift: middle raised surface: smooth and bright texture: moist
Conclusion:	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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