Shigella flexneri|138608 |BNCC

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Shigella flexneri

Subculture procedure	① Prepare 1 test tube containing 5-10mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to shatter it; break into pieces with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2 mL bacterial suspension onto plates, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once growth appears.
Growth conditions	37°C; 24-48h; aerobic
Storage conditions	2-8°C
Safety level	2
morphology	Colony diameter: 1-2 mm, circular, with neat edges; opaque, grayish-white on top, centrally convex, smooth surface, glossy appearance, moist texture, easily liftable; G- (red), short rod-shaped; Purity: Pure
Sharing mode	Public welfare sharing

Shigella flexneri

Storage conditions : 2~8 ℃

No. : 138608

Product format: freeze dried,200ul

Validity period : lyophilized tube for 6 years

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Recovery/subculture :37°C, aerobic, nutrient gravy medium. Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH7.0. 121 ℃,15min.

Recovery steps:

(1) Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;

(2) Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability:	good viability, in 40h NB bacterial fluid become turbid, bacterial fluid scribing plate colonies are obviously typical
colony morphology:	size: large color: light yellow shape: round edge: neat wet and dry: wet and smooth: smooth transparency: opaque uplift: slightly convex
Conclusion:	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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