BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC352246 |
| Description | R2A Agar |
| Composition | Peptone:0.5g, Casein Hydrolysate:0.5g, Yeast Extract:0.5g, Soluble Starch:0.5g, K2HPO4:0.3g, MgSO4:0.024g, Sodium Pyruvate(C3H3NaO3):0.3g, Agar:15.0g, Glucose:0.5g, pH:7.2±0.2(25℃) |
| Growth conditions | 30°C; 24-48h; aerobic |
| Subculture procedure | ① Prepare 1-2 fresh plates as described above; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to break it, and subsequently crush it with forceps; ③ Pipette 0.5mL of liquid medium into a freeze-dried vial, dissolve thoroughly, then return to the liquid test tube and mix well; ④ Pipette 0.2mL bacterial suspension onto plates, spread evenly; repeat twice to obtain two plates; ⑤ Incubate under specified conditions; strain is ready upon growth. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter 1-2mm, circular, with neat edges; opaque, grayish-white upper surface; central elevation; smooth, glossy surface; moist texture; easily lifted; G- (red); bacillus; purity: pure |
| Sharing mode | Public welfare sharing |
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