Acetylmicrobacterium|139356 |BNCC

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Exiguobacterium acetylicum

Subculture procedure	① Prepare 1 tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to shatter it; break into pieces with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid tube and mix thoroughly; ④ Pipette 0.2mL of the bacterial suspension onto a Agar plate and spread evenly. Repeat twice to obtain two plates. ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the bacterial strain grows.
Growth conditions	30°C; 18-24h; aerobic;
Storage conditions	2-8°C
Safety level	1
morphology	Size: Medium Color: Yellow Shape: Circular Edge: Irregular Moisture: Moist Surface: Smooth Transparency: Opaque Elevation: Raised
Sharing mode	Public welfare sharing

Exiguobacterium acetylicum

Storage conditions: 2~8 ℃

No. : 139356

Product format : freeze dried,200ul

Validity period : freezed-dried tube for 6 years

Biosafety level : 1,handle in ultra-clear table or safety cabinet

Reeceiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Recovery/subculture :30°C, aerobic, nutrient gravy medium. Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH7.0. 121 ℃,15min.

Recovery steps:

(1) Prepare 1 bottle of liquid medium or 2 plates;

(2) Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5mL of liquid medium into the freeze dried ampoule,make the strain pellet fully dissolved. transfer the liquid suspension to the liquid media, and culture in plate shaker at 30℃ for 18-24 hours (140r/min); or directly dispense 200ul of the liquid suspension into a agar plate evenly, then put the plates in incubator at 30℃ for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in 20h liquid medium become turbid, bacterial liquid marking plate colonies are obviously typical
colony morphology:	size: general color: yellow shape: round edge: irregular wet and dry: wet and smooth: smooth transparency: opaque uplift: uplift
Conclusion:	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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