Lactobacillus crudus Yeast|392382 |BNCC

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Kluyveromyces lactis

Culture medium	BNCC352251
Description	YM agar medium
Composition	Yeast Extract Powder:3.0g, Malt Extract:3.0g, Glucose:10.0g, Casein Peptone:5.0g, Agar:20.0g, pH:6.2±0.2(25℃)
Growth conditions	28°C; 24-48h; aerobic
Subculture procedure	① Prepare 1-2 plates as above; ② Disinfect the ampoule surface, open it in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it. Immediately break it open with forceps; ③ Pipette 0.5mL sterile water into the freeze-dried vial. After fully dissolving the powder, dispense 200μL/well onto plates and spread evenly; ④ Incubate plates under specified conditions until colonies emerge.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 2-4 mm; circular shape with neat margins; opaque, creamy-white upper surface; central elevation; smooth, glossy surface; moist texture; easily liftable; G+ (blue-violet); ovoid shape; purity: pure
Sharing mode	Public welfare sharing