Aspergillus oryzae|361469 |BNCC

Essential Information Certificate Related Products

Aspergillus oryzae

Subculture procedure	① Prepare 1-2 plates as described above; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to break it open; subsequently crush it with forceps; ③ Pipette 0.5mL sterile water into the freeze-dried vial, dissolve the powder thoroughly, then dispense approximately 200μL onto each of two plates, spreading evenly; ④ Incubate plates under specified conditions until colonies emerge for use.
Growth conditions	28°C; 5-7 days; aerobic
Storage conditions	2-8°C
Safety level	1
morphology	Small filamentous fungus; distinct colonies on PDA medium; white, spreading mycelium; produces dark yellow spores later; medium underside turns earthy yellow.
Sharing mode	Public welfare sharing

Aspergillus oryzae

Storage conditions: 2~8 ℃

No. 361469

Product format: agar slant in 14mm test tube

Validity period: growing culture, in 30 days

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The agar slant can be used directly. In principle, it can be used many times without contamination within the validity period, but viability will gradually decrease with time. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28 ℃, aerobic, PDA,5-7 days. PDA: potato boiling solution 1.0L, glucose 20.0g, agar 15.0g (not included in liquid medium), natural pH. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Recovery steps:

(1)Prepare 1-2 of above mentioned plates;

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4)Put the plates under the above culture conditions for cultivation

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 5 days plate colony is obvious
colony morphology:	Small filamentous fungi, with obvious colonies on PDA medium, and white hyphae, Spread growth, dark yellow spores in the later stage, khaki on the back of the medium
Conclusion:	good viability, no abnormal colony morphology, qualified

Download certificate