Pasteur Yeast|140792 |BNCC

Essential Information Certificate Related Products

Saccharomyces pasteurianus

Subculture procedure	① Prepare 1-2 plates as above; ② Open in a safety cabinet, cauterize the top with a spirit lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL sterile water into the freeze-dried vial, dissolve the powder thoroughly, then dispense 200μL/well onto plates, spreading evenly; ④ Incubate plates under the specified conditions; the culture is ready for use once growth appears.
Growth conditions	28°C; 24-48h; aerobic;
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 0.5-1 mm; circular with irregular margins; opaque; milky white on top; centrally convex; smooth surface; glossy appearance; moist texture; viscous consistency; G+ (blue-violet); cocci; Purity: Pure
Sharing mode	Public welfare sharing

Saccharomyces pasteurianus

Storage conditions: 2~8 ℃

No. 140792

Product format: freeze dried, 200ul

Validity : 6 years

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 30 ℃, aerobic, YM medium, 24-48h. YM medium: yeast extract 3.0g, malt extract 3.0g, glucose 10.0g, peptone 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 6.2±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:
(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;
(2) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;
(4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;
(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 48hours liquid medium become turbid,strain layer is obvious;colony is typical on the streaked plate
colony morphology: (above)	size: 1-2mm ;Shape: round ; Edge: neat ;Transparency: opaque color: milky white ;Protuberance: middle bulge ;Surface: smooth ; Texture: moist and easy to stir
Conclusion:	good viability, no abnormal colony morphology, qualified

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