Yellow lipophilic bacillus|360213 |BNCC

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Corynebacterium lipophiloflavum

Subculture procedure	① Prepare 1-2 plates as above; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL sterile water into the lyophilized vial, dissolve the bacterial powder completely, then dispense approximately 200μL onto each of two plates; spread evenly; ④ Incubate plates under specified conditions until colonies emerge.
Growth conditions	37°C; 18-24h; aerobic
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 0.5–1 mm; circular, with neat, opaque edges; grayish-white upper surface; central convexity; glossy, smooth surface; moist texture; G+ (blue-violet); bacillus
Sharing mode	Public welfare sharing

Corynebacterium lipophiloflavum

Storage conditions: 2~8 ℃

No. 360213

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Culture conditions:30 ℃,aerobic, Columbia blood plate (ready-to-use type),18-24h.

Recovery steps:

①Prepare 1-2 of above mentioned plates;

②Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

③Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

④Put the plates under the above culture conditions for cultivation 18-24h.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability,in24hours strain layer become obvious, colony is typical on the streaked plate
Colony morphology: (pictured above)	Size: 0.5-1mm Shape: Round Edge: Neat Transparency: Opaque Color: off-white Protuberance: Middle convex surface: bright and smooth texture: moist
conclusion: good viability, no abnormal colony morphology, qualified

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