White-covered black egg nest|150431 |BNCC

Essential Information Certificate Related Products

Cyathus pallidus

Subculture procedure	① Prepare 1-2 plates as above; ② Disinfect the surface of test tube slants before opening in a biosafety cabinet; ③ Cut blocks (see appendix) into 0.5×0.5 cm² squares using a sterile inoculating fork and spatula; ④ Place the small square block flat onto the center of the Agar plate, on the agar surface; ⑤ Incubate the Agar plate upright under the specified conditions; the strain is ready for use once growth appears.
Growth conditions	28°C; 7-10 days; aerobic;
Storage conditions	2-8°C
Safety level	1
morphology	Small filamentous fungus; distinct colonies on comprehensive PDA medium; mycelium on the Agar plate surface is grayish-white, spreading toward the Agar plate edge; underside of the medium is yellow.
Sharing mode	Public welfare sharing

Cyathus pallidus

Storage conditions : 2~8 ℃

No. : 150431

Product format :agar slant in 14mm test tube

Validity : growing culture, in 30 days

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28 ℃, aerobic, integrated PDA,7-10 days. Comprehensive PDA: potato boiling solution 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 *7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0± 0.2. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm²;

(4) lay flat the small pieces to the center of the agar plate;

(5)put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 7days strain layer become obvious
colony morphology:	small filamentous fungi, the colony is obvious on the comprehensive PDA medium, the mycelium of the plate layer is gray-white, spreading to the edge of the plate, and the back of the medium is yellow.
Conclusion:	good viability, no abnormal colony morphology, qualified

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