Fetal Campylobacter subspecies of sexually transmitted diseases|360236 |BNCC

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Campylobacter fetus subsp. venerealis

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37 ℃; 5-7 days; Micro aerobic (3% -5% O2, 10% CO2);
Subculture procedure	① Prepare one of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of Brucella broth and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate and apply evenly; Cover the agar surface with sufficient (1-4ml) Brucella broth or brain extract broth to maintain biphasic culture throughout the entire cultivation period (the culture will not grow on a dry surface). ④ Transfer to the above cultivation conditions for cultivation.
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 0.5-1mm, circular, with neat edges, semi transparent, protruding in the middle, smooth surface, G - (red), rod-shaped, purity: pure
Sharing mode	Public welfare sharing