Rust-Swimming Actinomycetes|358496 |BNCC

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Actinoplanes ferrugineus

Culture medium	BNCC362653
Description	GYM Streptomyces Agar
Composition	Glucose:4.0g, Yeast Extract:4.0g, Malt Extract:10.0g, CaCO₃:2.0g, Agar:15.0g, pH:7.2(25℃)
Growth conditions	28°C; 48-72h; aerobic
Subculture procedure	① Prepare two plates as above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then promptly add sterile water to rupture. Crush ampoule with forceps; ③ Pipette 0.5 mL sterile water into lyophilized tube. Thoroughly dissolve bacterial powder, then inoculate two plates (200 μL/Agar plate) with uniform spread; ④ Incubate plates under specified conditions until colonies emerge.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm; irregular, opaque edges; white upper surface with central elevation; rough texture; dry consistency; difficult to pick/cut; Gram-positive (blue-violet); bacilli; purity: pure
Sharing mode	Public welfare sharing