Helicobacter pylori|359033 |BNCC

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Helicobacter pylori

Subculture procedure	① Prepare 1-2 fresh plates as mentioned above; ② Dissolve the frozen storage tube in a 37 ℃ water bath, shake quickly to dissolve, wipe the outer wall of the frozen storage tube with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, pour it into 1-2 agar plates, and apply evenly; ④ Cover the agar surface with sufficient (1-4ml) tryptone soy broth medium to maintain a liquid film throughout the entire cultivation period, and prevent the culture from growing on dry surfaces. ⑤ Place the agar plate under the above cultivation conditions for cultivation.
Growth conditions	37 ℃; 3-4 days; 5% CO2
Storage conditions	-80 ℃
Safety level	2
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth on the surface, moist texture, easy to pick up, G - (red), Vibrio species
Sharing mode	Public welfare sharing

Helicobacter pylori

Storage conditions: 2~8 ℃

No. 359033

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Culture conditions:37 ℃,5% CO₂, Columbia blood plate (ready-to-use type),48-72h.

Recovery steps:

①Prepare 1-2 of above mentioned plates;

②Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

③Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

④Put the plates under the above culture conditions for cultivation，and the strain can be used when it grows out.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 72h obvious strain layer occurs on the plate; colony is typical
Colony morphology: (as shown above)	size: 1-2mm ; Shape: round ; Edge: neat ; Transparency: translucent ; Color: off-white uplift: middle bulge ; Surface: gray and smooth ; Texture: moist and easy to stir
conclusion: good viability, no abnormal colony morphology, qualified

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