Yersinia enterocolitis|336699 |BNCC

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Yersinia enterocolitica

Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	29 ℃; 18-24h; aerobic
Storage conditions	2-8 ℃
Safety level	2
morphology	Size: General Shape: Circular Edge: Neat Edge Transparency: Opaque Color: Grey White Rise: Raised Surface: Smooth Texture: Moist
Sharing mode	Public welfare sharing

Yersinia enterocolitica

Storage conditions : 2~8 ℃

No. : 336699

Product Format: freeze dried, 200ul

Validity period: 6 years

Biosafety level : 2,handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :28°C, aerobic, nutritious agar/gravy medium (NA/NB). Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH7.0. 121 ℃, 15min.

Recovery steps:

(1)Prepare a flask of NB liquid media or two NA agar plates.

(2)Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid medium into the freeze dried ampoule,make the strain pellet fully dissolved. transfer the liquid suspension to the liquid media, and culture in plate shaker at 37℃ for 18-24 hours (140r/min); or directly dispense 200ul of the liquid suspension into a NA agar plate evenly, then put the plates in incubator at 37℃ for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in 20h NB liquid medium become turbid, NA strain layer is obvious
colony morphology: (above)	size: general shape: round edge: neat edge transparency: opaque color: off-white uplift: raised surface: smooth texture: moist
conclusion	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified & nbsp;

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