Brisbane Mycobacterium|356299 |BNCC

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Mycobacterium brisbanense

Culture medium	BNCC360851
Description	Middlebrook 7H10 Agar
Composition	(NH₄)₂SO₄:0.5g, KH₂PO₄:1.5g, Na₂HPO₄:1.5g, Na₃C₆H₅O₇:0.4g, MgSO₄:0.025g, CaCl₂:0.5mg, ZnSO₄:0.001g, CuSO₄:0.001g, Sodium L-Glutamate(C₅H₈NNaO₄):0.5g, C₆H₈FeNO₇:0.04g, Pyridoxine Hydrochloride(C₈H₁₁NO₃.HCl):0.001g, Biotin(C₁₀H₁₆N₂O₃S):0.5mg, Malachite Green:0.25mg, OADC Supplement:10%(added at 50℃), Glycerol:5ml, Agar:15.0g, pH:6.4-6.8(25℃)
Growth conditions	37°C; 3-5 days; aerobic
Subculture procedure	① Prepare 1-2 plates as described above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then promptly add sterile water to rupture. Crush with forceps; ③ Pipette 0.5mL sterile water into lyophilized tube. After complete dissolution, dispense 200μL/well onto plates and spread evenly; ④ Incubate plates under specified conditions until colonies emerge.
Storage conditions	2-8°C
Safety level	2
morphology	Colony diameter: 1-2 mm; circular shape with neat margins; opaque, pale white upper surface; central elevation; smooth texture; moist consistency; easily liftable; non-pigmented; Gram-positive (blue-violet); bacillus; purity: pure
Sharing mode	Public welfare sharing