Brisbane Mycobacterium|356299 |BNCC

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Brisbane Mycobacterium-BNCC
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  • Mycobacterium brisbanense-BNCC

Mycobacterium brisbanense

  • Price： $ 223
  • number：BNCC356299
  • MPs：
    Colony diameter: 1-2 mm; circular shape with neat margins; opaque, pale white upper surface; central elevation; smooth texture; moist consistency; easily liftable; non-pigmented; Gram-positive (blue-violet); bacillus; purity: pure
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Mycobacterium brisbanense
Culture medium BNCC360851
Description Middlebrook 7H10 Agar
Composition (NH4)2SO4:0.5g, KH2PO4:1.5g, Na2HPO4:1.5g, Na3C6H5O7:0.4g, MgSO4:0.025g, CaCl2:0.5mg, ZnSO4:0.001g, CuSO4:0.001g, Sodium L-Glutamate(C5H8NNaO4):0.5g, C6H8FeNO7:0.04g, Pyridoxine Hydrochloride(C8H11NO3.HCl):0.001g, Biotin(C10H16N2O3S):0.5mg, Malachite Green:0.25mg, OADC Supplement:10%(added at 50℃), Glycerol:5ml, Agar:15.0g, pH:6.4-6.8(25℃)
Growth conditions 37°C; 3-5 days; aerobic
Subculture procedure ① Prepare 1-2 plates as described above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then promptly add sterile water to rupture. Crush with forceps; ③ Pipette 0.5mL sterile water into lyophilized tube. After complete dissolution, dispense 200μL/well onto plates and spread evenly; ④ Incubate plates under specified conditions until colonies emerge.
Storage conditions 2-8°C
Safety level 2
morphology Colony diameter: 1-2 mm; circular shape with neat margins; opaque, pale white upper surface; central elevation; smooth texture; moist consistency; easily liftable; non-pigmented; Gram-positive (blue-violet); bacillus; purity: pure
Sharing mode Public welfare sharing
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