Helicobacter pylori|356182 |BNCC

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Helicobacter pylori

Subculture procedure	① Dissolve the Frozen vial tube in a 37 ℃ water bath and shake quickly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (keep the plates moist during the cultivation process, which can be opened in a safe cabinet and added with an appropriate amount of tryptone soy broth); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	37 ℃; 3-4 days; 5% CO2
Storage conditions	-80 ℃
Safety level	2
morphology	The colony diameter is 0.5-1mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth on the surface, moist texture, easy to pick up, G - (red), spiral shaped
Sharing mode	Public welfare sharing

Helicobacter pylori

Storage conditions: 2~8 ℃

Number: 356182

Product format: 90mm plate

Validity period: growing culture, in 10 dyas

Format level: 2 , handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃,10% CO2, Columbia blood plate (ready-to-use type),3-5 days.

Recovery steps:
(1) Prepare 1-2 of above mentioned plates;
(2) Disinfect the surface of the plate and open it in a safety cabinet;
(3) The plates were eluted with sterile water, and the bacterial suspension was absorbed and transferred to a new medium;
(4) Put the plates under the above culture conditions for cultivation for 3-5days.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test result
viability:	good viability, in 4 days strain layer is obvious;colony is typical on the streaked plate
colony morphology: (above)	size: 0.5-1mm shape: round edge: neat transparency: opaque color: off-white bulge: middle raised surface: bright and smooth texture: moist and easy to stir up
conclusion:	good viability, no abnormal colony morphology, qualified

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