Nitrosomonas macrophylla|357823 |BNCC

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Azomonas macrocytogenes

Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	28 ° C; 24-48 hours; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, round, with neat edges, opaque, yellow on the front, light color, raised in the middle, smooth surface, moist texture, G - (red), oval shape, purity: pure
Sharing mode	Public welfare sharing

Azomonas macrocytogenes

Storage conditions : 2~8 ℃

No. : 357823

Product format :freeze dried,200ul

Validity period : 6 years

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :37℃, aerobic, nitrogen-fixing medium, 24-48h. Nitrogen-fixing medium: KH2PO4 0.2g, K2HPO4 0.8g, MgSO4 7H2O 0.2g, CaSO4 2H2O 0.1g, FeCl3 trace g, Na2MoO4 2H2O trace g, yeast extract 0.5g, mannitol 20.0g, agar 15.0g, distilled water 1.0L, pH 7.2. Sterilize at 121°C for 15min.

Recovery steps:

(1) Prepare 1-2 of above mentioned plates；

(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 48h liquid medium become turbid, obvious strain layer occurs on the plate; colony is typical on marked plate
colony morphology (above)	size: 1-2mm shape: round edge: neat transparency: opaque color: yellow uplift: middle raised surface: bright and smooth texture: moist and easy to stir
conclusion	good viability, no abnormal colony morphology, qualified

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