Anomalous Rhizopus|356130 |BNCC

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Absidia anomala

Subculture procedure	① Prepare 1-2 plates as above; ② Open in a biosafety cabinet, cauterize the top with an alcohol lamp, then promptly add sterile water to rupture it. Use forceps to break it into pieces; ③ Pipette 0.5mL sterile water into the lyophilized tube. After fully dissolving the powder, dispense approximately 200μL onto each of two plates and spread evenly; ④ Incubate plates under specified conditions until colonies emerge.
Growth conditions	28°C; 5-7 days; aerobic;
Storage conditions	2-8°C
Safety level	1
morphology	Small filamentous fungi produce distinct colonies on PDA medium. Mycelium appears white, cottony, dense, and vigorous, spreading toward Agar plate edges to cover the entire surface. The underside of the medium turns pale yellow.
Sharing mode	Public welfare sharing

Absidia anomala

Storage conditions: 2~8 ℃

No. 356130

Product format: freeze dried, 200ul

Validity : 6 years

Biosafety level: 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28 ℃, aerobic, comprehensive PDA,5-7 days, comprehensive PDA: potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Recovery steps:
(1) Prepare 1-2 of above mentioned plates;
(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4) Put the plates under the above culture conditions for cultivation for 5-7 days.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability， in 6days strain layer is obvious
colony morphology: (above)	Small filamentous fungi have obvious colonies on the integrated PDA medium, with white hyphae, cotton wool-like, dense and vigorous, spreading and growing to the edge of the plate, covering the plate, and the back of the medium is light yellow.
Conclusion:	good viability, no abnormal colony morphology, qualified

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