Mutant Streptococcus|186173 |BNCC

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Streptococcus mutans

Subculture procedure	① Prepare 2 plates; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to break it open; use forceps to crush it; ③ Pipette 0.5mL sterile water into the freeze-dried vial and mix thoroughly; ④ Pipette 0.2mL of the bacterial suspension onto the Agar plate and spread evenly; repeat twice to obtain two plates; ⑤ Incubate all plates under specified conditions until colonies develop.
Growth conditions	37°C; 24-48h; aerobic
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm; circular, irregularly margined, opaque; upper surface white, centrally convex, glossy, dry texture; G+ (blue-violet), cocci; purity: pure
Sharing mode	Public welfare sharing

Streptococcus mutans

Storage conditions: 2~8 ℃

No. 186173

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 37℃, 5%CO2, Columbia blood plate (ready-to-use), 24-48h.

Recovery steps:

①Prepare 1-2 of above mentioned plates;

②Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

③Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

④Put the plates under the above culture conditions for cultivation 5-7days.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability，in 48h strain layer become obvious, colony is typical
colony morphology: (above)	size: 1-2mm ; Shape: round ; Edge: irregular ; Transparency: opaque color: white ; Protuberance: middle bulge ; Surface: bright ; Texture: dry
Conclusion:	good viability, no abnormal colony morphology, qualified

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