Streptococcus equinus

No. 186309

Storage conditions: 2~8 ℃

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clean table or safety cabinet

Receving notices:if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃, aerobic, BHI: 4.0g of bovine brain extract powder, 4.0g of bovine heart extract powder, 5.0g of peptone, 16.0g of casin peptone, 5.0g of NaCl, 2.0g of glucose, 2.5g of disodium hydrogen phosphate, pH7.4±0.2. 121 ℃,15min.

Recovery steps:

(1)Prepare a flask of BHI liquid media or two BHI agar plates.

(2)Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; 

(3)Draw 0.5mL of liquid medium into the freeze dried ampoule,make the strain pellet fully dissolved. transfer the liquid suspension to the liquid media, and culture in plate shaker   at 37℃ for 18-24 hours (140r/min); or directly dispense 200ul of the liquid suspension into a BHI agar plate evenly, then put the plates in incubator at 37℃ for 24-48 hours.

Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows: