Resinous Branch Spore Fungi|186386 |BNCC

Essential Information Certificate Related Products

Hormoconis resinae

Subculture procedure	① Prepare 1 test tube containing 5-10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL liquid medium into the freeze-dried vial, dissolve thoroughly, then return to the liquid test tube and mix well; ④ Pipette 0.2mL bacterial suspension onto plates, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the strain grows.
Growth conditions	28°C; 5-7 days; aerobic;
Storage conditions	2-8°C
Safety level	1
morphology	Small filamentous fungus. On PDA medium, colonies are distinct, deep gray, dense and vigorous, producing spores. The underside of the medium appears pale yellow.
Sharing mode	Public welfare sharing

Hormoconis resinae

Storage conditions: 2~8 ℃

No. 186386

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28℃, aerobic, PDA, 5-7 days, PDA: 1.0L potato cooking liquid, 20.0g glucose, 15.0g agar (not included in liquid medium), natural pH. Sterilize at 121°C for 15min. Potato cooking liquid: Weigh 200g peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Recovery steps:

①Prepare 1-2 of above mentioned plates;

②Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

③Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

④Put the plates under the above culture conditions for cultivation 5-7days.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 5-7days strain layer become obvious
colony morphology: (above)	small filamentous fungi, with obvious colonies on PDA medium, dark gray, dense and vigorous, sporulating, light yellow on the back of the medium
Conclusion:	good viability,no abnormal colony morphology, qualified

Download certificate