BeNa Culture Collection
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| Culture medium | BNCC352233 |
| Description | Potato glucose agar |
| Composition | Potato Infusion Powder:6.0g, Glucose:20.0g, Agar:20.0g, pH:5.6±0.2(25℃) |
| Growth conditions | 28°C; 5-7 days; aerobic; |
| Subculture procedure | ① Prepare 1 test tube containing 5-10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL liquid medium into the freeze-dried vial, dissolve thoroughly, then return to the liquid test tube and mix well; ④ Pipette 0.2mL bacterial suspension onto plates, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the strain grows. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Small filamentous fungus. On PDA medium, colonies are distinct, deep gray, dense and vigorous, producing spores. The underside of the medium appears pale yellow. |
| Sharing mode | Public welfare sharing |
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