Bacillus subtilis|357806 |BNCC

Essential Information Certificate Related Products

Bacillus pumilus

Subculture procedure	① Prepare 1-2 plates as above; ② Open in a biosafety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL sterile water into the lyophilized vial, dissolve the powder completely, then dispense 200 μL/well onto plates, spreading evenly; ④ Incubate plates under the specified conditions; the culture is ready for use once growth appears.
Growth conditions	30°C; 18-24h; Aerobic;
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm; circular, with neat edges; opaque, grayish-white upper surface; central elevation; smooth, glossy surface; moist texture; easily liftable; G+ (blue-violet); bacillus; purity: pure
Sharing mode	Public welfare sharing

Bacillus pumilus

Storage conditions : 2~8 ℃

No.: 357806

Product format : freeze dried,200ul

Validity period: 6 years

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :30 ℃, aerobic, nutrient agar medium, 24h. Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH7.0. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1) Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;

(2) Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 24h liquid medium become turbid, obvious strain layer occurs on the plate; colony is typical on marked plate
colony morphology (above)	size: 2-4mm shape: round edge: irregular transparency: opaque color: off-white uplift: flat surface: rough texture: dry
conclusion	good viability, no abnormal colony morphology, qualified

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