Salmonella enterica subspecies enterica|186362 |BNCC

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Salmonella enterica subsp. enterica serovar Paratyphi-B

Culture medium	BNCC352249
Description	Malt extract agar
Composition	Malt Extract Powder:130.0g, Chloramphenicol:0.1g, Agar:15.0g, pH:6.0±0.2(25℃)
Growth conditions	37°C; 18-24h; Aerobic;
Subculture procedure	① Prepare 1 tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to break it; subsequently crush it with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried vial, dissolve thoroughly, then return to the liquid tube and mix well; ④ Pipette 0.2mL of the bacterial suspension onto each plate and spread evenly. Repeat twice to obtain two plates. ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once bacterial growth appears.
Storage conditions	2-8°C
Safety level	2
morphology	Size: Medium Color: Grayish-white Shape: Circular Edge: Neat Moisture: Moist Surface: Smooth Opacity: Opaque Elevation: Raised
Sharing mode	Public welfare sharing