BeNa Culture Collection
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| Culture medium | BNCC352249 |
| Description | Malt extract agar |
| Composition | Malt Extract Powder:130.0g, Chloramphenicol:0.1g, Agar:15.0g, pH:6.0±0.2(25℃) |
| Growth conditions | 37°C; 18-24h; Aerobic; |
| Subculture procedure | ① Prepare 1 tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to break it; subsequently crush it with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried vial, dissolve thoroughly, then return to the liquid tube and mix well; ④ Pipette 0.2mL of the bacterial suspension onto each plate and spread evenly. Repeat twice to obtain two plates. ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once bacterial growth appears. |
| Storage conditions | 2-8°C |
| Safety level | 2 |
| morphology | Size: Medium Color: Grayish-white Shape: Circular Edge: Neat Moisture: Moist Surface: Smooth Opacity: Opaque Elevation: Raised |
| Sharing mode | Public welfare sharing |
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