Salmonella enterica subspecies enterica|186362 |BNCC

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Salmonella enterica subsp. enterica serovar Paratyphi-B

Subculture procedure	① Prepare 1 tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to break it; subsequently crush it with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried vial, dissolve thoroughly, then return to the liquid tube and mix well; ④ Pipette 0.2mL of the bacterial suspension onto each plate and spread evenly. Repeat twice to obtain two plates. ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once bacterial growth appears.
Growth conditions	37°C; 18-24h; Aerobic;
Storage conditions	2-8°C
Safety level	2
morphology	Size: Medium Color: Grayish-white Shape: Circular Edge: Neat Moisture: Moist Surface: Smooth Opacity: Opaque Elevation: Raised
Sharing mode	Public welfare sharing

Salmonella paratyphi β

Storage conditions : 2~8 ℃

No.: 186362

Product format :freeze dried,200ul

Validity period : freezed-dried tube for 6 years

Biosafety level: 2, handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Recovery/subculture:37°C, aerobic, nutrient gravy medium. Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH7.0. 121 ℃,15min.

Recovery steps:

(1) Prepare 1 bottle of liquid medium or 2 plates;

(2) Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5mL of liquid medium into the freeze dried ampoule,make the strain pellet fully dissolved. transfer the liquid suspension to the liquid media, and culture in plate shaker at 37℃ for 18-24 hours (140r/min); or directly dispense 200ul of the liquid suspension into a agar plate evenly, then put the plates in incubator at 37℃ for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability:	good viability, in 24 hours NB bacterial fluid become turbid, bacterial fluid scribing plate colonies are obviously typical
colony morphology:	size: general color: off-white shape: round edge: neat wet and dry: wet and smooth: smooth transparency: opaque uplift: uplift
Conclusion:	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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