Aspergillus niger|186602 |BNCC

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Aspergillus niger

Subculture procedure	① Prepare 1 test tube containing 5-10 mL liquid medium and 2 plates; ② Open in a biosafety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL of the bacterial suspension onto a plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once growth appears.
Growth conditions	28°C; 5-7 days; Aerobic;
Storage conditions	2-8°C
Safety level	1
morphology	Fungal filaments form distinct colonies on PDA medium, with vigorous mycelial growth. Initial mycelium appears white, occasionally tinged yellowish-brown, and densely packed. Later stages produce dark brown spores.
Sharing mode	Public welfare sharing

Aspergillus niger

Storage conditions: 2~8 ℃

No. 186602

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 25-28℃, aerobic, integrated PDA, 5-7 days. Comprehensive PDA: Potato cooking liquid 1.0L, glucose 20.0g, KH2PO4 3.0g, MgSO4·7H2O 1.5g, trace amount of vitamin B1, agar 20.0g, pH 6.0±0.2. Sterilize at 121℃ for 15min. Potato cooking liquid: Weigh 200g of peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Recovery steps:

① Prepare above 2 pieces of plates;

② Sterilizing the ampoule, open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

③ Draw 0.5mL of liquid culture medium into the freeze dried ampoule,make the strain pellet fully dissolved and inoculate a plate with the solution, then distribute it well;

④ Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

⑤ Put the agar plates under the above culture conditions for cultivation，the strain can be used when it grow out.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability:	good viability, in 5-7days strain layer become obvious;colony is typical on the streaked plate
colony morphology: (above)	filamentous fungi, the colony is obvious on the comprehensive PDA medium, the hyphae are vigorous and spread, the initial hyphae are white, some are slightly yellowish brown, dense, and the late hyphae produce brown and black spores.
Conclusion:	good viability, no abnormal colony morphology, qualified

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