Aspergillus oryzae|185853 |BNCC

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Aspergillus oryzae

Subculture procedure	① Prepare 1-2 tablets mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	28°C; 5-7 days; aerobic;
Storage conditions	2-8°C
Safety level	1
morphology	Filamentous fungi produce distinct colonies on malt extract agar, with vigorous mycelial growth forming an uneven surface. Initially, mycelium appears dense and white; later, yellow-green spores develop along the edges.
Sharing mode	Public welfare sharing

Aspergillus oryzae

Storage conditions: 2~8 ℃

No. 185853

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 25-28℃, aerobic, wort agar, 5-7 days. Wort agar: malt extract 20.0g, glucose 20.0g, peptone 1.0g, agar 20.0g, distilled water 1.0L, pH natural. Sterilize at 121°C for 15min.

Recovery steps:

① Prepare above 2 pieces of plates;

② Sterilizing the ampoule, open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

③ Draw 0.5mL of liquid culture medium into the freeze dried ampoule,make the strain pellet fully dissolved and inoculate a plate with the solution, then distribute it well;

④ Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

⑤ Put the agar plates under the above culture conditions for cultivation，the strain can be used when it grow out.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability：	good viability, in 7days strain layer become obvious
colony morphology: (above)	filamentous fungi, with obvious colonies on wort agar, vigorous spread and growth of hyphae, uneven surface, white and dense hyphae at the initial stage, and yellow-green spores at the edge at the later stage.
Conclusion:	good viability, no abnormal colony morphology, qualified

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