Fig Aspergillus|225981 |BNCC

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Aspergillus ficuum

Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in the safety cabinet,
Growth conditions	28 ℃, 3-5 days, aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	A small filamentous fungus forms distinct colonies on comprehensive PDA medium, initially with white, spreading mycelium that later produces yellow-green spores; the reverse side of the medium appears khaki-colored.
Sharing mode	Public welfare sharing

Aspergillus ficuum

BNCC No.：225981

Biosafety level：4

2.Storage conditions：

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1. Comprehensive PDA agar: potato boiling solution 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0 ±0.2.

2. Atmosphere：aerobic

3. Temperature：25-28 ℃,4 days

4.Notes：

1.Normal culturing time：24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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