BeNa Culture Collection
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| Culture medium | BNCC352250 |
| Description | Comprehensive PDA agar |
| Composition | Potato Infusion Powder:10.0g, Glucose:20.0g, KH2PO4:3.0g, MgSO4.7H2O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃) |
| Growth conditions | 28°C; 5-7 days; aerobic |
| Subculture procedure | ① Prepare 1-2 plates as described above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then quickly add sterile water to rupture. Immediately crush with forceps; ③ Pipette 0.5 mL sterile water into lyophilized tube. After complete dissolution of powder, dispense 200 μL/well onto plates and spread evenly; ④ Incubate plates under the specified conditions. The culture is ready for use once bacterial growth appears. |
| Storage conditions | 2-8°C |
| Safety level | 2 |
| morphology | Spreading growth pattern, produces yellow spores. Top surface yellow, underside yellow. Purity: Pure |
| Sharing mode | Public welfare sharing |
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