Streptomyces genus|335904 |BNCC

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Streptomyces sp.

Subculture procedure	① Prepare 1 tube containing 5-10mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL liquid medium into the freeze-dried vial, dissolve completely, then transfer back to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL of the bacterial suspension onto a Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once growth appears.
Growth conditions	28°C; 24-48h; aerobic
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm, circular, with neat edges; opaque, grayish-white on top, flat in shape, rough surface, glossy appearance, firm texture, difficult to pick/cut; Gram-positive (blue-violet), filamentous; Purity: Pure
Sharing mode	Public welfare sharing

Streptomyces sp.

Description

1. BNCC No.: 335904

2. Biosafety level: 4

Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

Growth Conditions

1. Gao's synthetic agar: soluble starch 20.0g,KNO₃ 1.0g,K₂HPO₄ 0.5g,MgSO₄·7H₂O 0.5 g,NaCl 0.5g,FeSO₄ 0.01g, agar 15.0g, distilled water 1.0 L,pH 7.2-7.4. 121 ℃,15min.

2. Atmosphere: aerobic

3. Temperature: 28 ℃

Notes:
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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