Rhizopus niger|336302 |BNCC

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Rhizopus nigricans Ehrenberg

Subculture procedure	① Prepare 1 test tube containing 5–10 mL liquid medium and 2 plates; ② Open in biosafety cabinet, flame top with alcohol lamp, then quickly add sterile water to shatter; break into pieces with forceps; ③ Pipette 0.5 mL liquid medium into freeze-dried vial, dissolve completely, then return to liquid test tube and mix thoroughly; ④ Pipette 0.2 mL of the bacterial suspension onto each Agar plate and spread evenly. Repeat twice to obtain two plates. ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the bacterial strain grows.
Growth conditions	28°C; 5-7 days; aerobic;
Storage conditions	2-8°C
Safety level	1
morphology	Filamentous fungi produce distinct colonies on comprehensive PDA medium, exhibiting rapid growth. Hyphae are grayish-white, densely packed, tall, and spreading to cover the entire Agar plate. Later stages produce black spores.
Sharing mode	Public welfare sharing

Rhizopus nigricans

Storage conditions : 2~8 ℃

No. : 336302

Product format: freeze dried, 200ul

Validity : 6 years

Biosafety level: 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :28 ℃, aerobic, integrated PDA,3-5 days. Comprehensive PDA: potato boiling solution 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Recovery steps:
(1) Prepare 1-2 of above mentioned plates;
(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4) put the plates under the above culture conditions, and the strains can be used when they grow.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability,in 3 days strain layer is obvious
colony morphology: & nbsp;	filamentous fungi have obvious colonies and rapid growth on comprehensive PDA medium. hyphae are gray-white, dense and long, spread and grow to cover the entire plate, and produce black spores in the later period
Conclusion:	good viability, no abnormal colony morphology, qualified ;

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