Penicillium genus|336748 |BNCC

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Penicillium sp.

Subculture procedure	① Prepare 2 plates; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to break it open; use forceps to crush it; ③ Pipette 0.5mL sterile water into the freeze-dried vial, dissolve completely, and mix thoroughly; ④ Pipette 0.2mL of the bacterial suspension onto the agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all plates under the specified conditions. The culture is ready for use once bacterial growth appears.
Growth conditions	28°C; 5-7 days; aerobic
Storage conditions	2-8°C
Safety level	1
morphology	Top surface: light brown; bottom surface: yellow, spreading growth
Sharing mode	Public welfare sharing

1.Description

1.Name: Penicillium nalgiovense

2. BNCC No.:336748

3. Biosafety level: 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1. Comprehensive PDA:20% potato juice 1L, glucose 20g ,KH2PO4 3g, MgSO4.7H2O 1.5g, thiamine trace, agar 15g, pH 6

2. Atmosphere: aerobic

3, Temperature: 25-28 ℃

4.Notes:

1.Normal culturing time, 1-2days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and long-term storage is not recommended.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Users should ensure the safe storage and operation of the strain.Waste generated from the handling process should be discarded after high-pressure sterilization.

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