Penicillium resi|336886 |BNCC

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Penicillium raistrickii

Subculture procedure	① Prepare 1-2 plates as described above; ② Open plates in a safety cabinet after surface disinfection; ③ Pipette 1-3 ml sterile water onto the Agar plate; use a sterile spreader to scrape the surface culture layer back and forth to create a bacterial suspension; ④ Pipette the suspension with a Pasteur pipette onto fresh plates and spread evenly; ⑤ Incubate plates under the specified conditions.
Growth conditions	26°C; 5-7 days; aerobic
Storage conditions	2-8°C
Safety level	1
morphology	Filamentous fungi exhibit distinct colonies on comprehensive PDA medium, with vigorous, dense, and low-lying mycelium. Initial mycelium appears bluish-white, later producing grayish-green spores. May display sparse radial wrinkles or indistinct concentric rings.
Sharing mode	Public welfare sharing

1.Description

1, Name:Penicilliumitalicum

2. BNCC No.:336886

3. Biosafety level : 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1. Comprehensive PDA:20% potato juice 1L, glucose 20g ,KH2PO4 3g, MgSO4.7H2O 1.5g, thiamine trace, agar 15g,pH natural.

2. Atmosphere:aerobic

3. Temperature: 25-26 ℃

4.Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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