Listeria monocytogenes|336912 |BNCC

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Listeria innocua

Culture medium	BNCC352252
Description	Brain Heart Infusion Agar
Composition	Beef Brain Infusion Powder:4.0g, Beef Heart Infusion Powder:4.0g, Peptone:5.0g, Casein Peptone:16.0g, NaCl:5.0g, Glucose:2.0g, Na₂HPO₄:2.5g, Agar:13.5g, pH:7.4±0.2(25℃)
Growth conditions	37°C; 18-24h; aerobic
Subculture procedure	① Prepare 1-2 tablets mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm, circular, with neat margins, opaque, grayish-white on top, centrally convex, smooth surface, glossy appearance, moist texture, easily liftable, bacilli, Purity: Pure, G+ (blue-violet).
Sharing mode	Public welfare sharing