Lactobacillus plantarum|336981 |BNCC

Essential Information Certificate Related Products

Sporolactobacillus inulinus

Subculture procedure	① Prepare 1 test tube containing 5–10 mL liquid medium and 2 plates (pre-deoxygenated in anaerobic environment for 24 hours); ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break into pieces with forceps; ③ Pipette 0.5mL liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL bacterial suspension onto plates, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the strain grows.
Growth conditions	Culture temperature 37°C; Incubation time 2-3 days; anaerobic gas environment;
Storage conditions	2-8°C
Safety level	1
morphology	Size: 0.5-1mm Shape: Circular Edge: Irregular Transparency: Opaque Color: Milky white Elevation: Central dome Surface: Bright and smooth Texture: Moist and viscous
Sharing mode	Public welfare sharing

1.Description

1. Name: Sporolactobacillus inulinus

2. BNCC No.:336981

3. Biosafety level: 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1. MRS medium: cheese peptone 10.0g, beef powder 8.0g, yeast powder 4.0g, glucose 20.0g, magnesium sulfate 0.2g, sodium acetate 5.0g, triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.05g, Tween 80 1.0g, distilled water 1000mL. pH6.2±0.2.

2. Atmosphere: anaerobic

3, Temperature: 37 ℃,48h

4.Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

Download certificate