Enterococcus faecalis (Enterococcus faecalis)|337174 |BNCC

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Enterococcus faecalis

Subculture procedure	① Prepare 1 test tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to shatter it; break into pieces with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2 mL of the bacterial suspension onto each Agar plate and spread evenly. Repeat twice to obtain two plates. ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once bacterial growth appears.
Growth conditions	37°C; 18-24h; aerobic
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 0.5–1 mm; circular, with neat, opaque edges; grayish-white upper surface; raised center; smooth surface; easily liftable; Gram-positive (blue-violet); cocci
Sharing mode	Public welfare sharing

Enterococcus faecalis

Storage conditions: 2~8 ℃

No.: 337174

Product format: freeze dried, 200ul

Validity period: Freeze-dried tube for 6 years

Biosafety level: 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Recovery/subculture:37 ℃, aerobic, BHI: 4.0g of bovine brain extract powder, 4.0g of bovine heart extract powder, 5.0g of peptone, 16.0g of casin peptone, 5.0g of NaCl, 2.0g of glucose, 2.5g of disodium hydrogen phosphate, pH7.4 ± 0.2. 121 ℃,15min.

Recovery steps:

(1)Prepare one flask of BHI liquid medium or two BHI plates;

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid medium into the freeze dried ampoule,make the strain pellet fully dissolved. transfer the liquid suspension to the liquid media, and culture in a shaker at 37℃ (140r/min); or directly dispense 200ul of the liquid suspension into a BHI agar plate evenly, then put the plates in incubator at 37℃ for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability,in 20h BHI bacterial liquid is turbid, BHI strain layer is obvious
colony morphology	size: small color: milky white shape: round edge: neat edge dry and wet: wet smooth: smooth and bright transparency: opaque uplift: uplift
conclusion	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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