Trichoderma longibrachiatum|341613 |BNCC

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Trichoderma longibrachiatum

Subculture procedure	① Prepare 1 test tube containing 5-10mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL liquid medium into the freeze-dried vial, dissolve thoroughly, then return to the liquid test tube and mix well; ④ Pipette 0.2mL bacterial suspension onto plates, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once growth appears.
Growth conditions	28°C; 3-5 days; aerobic;
Storage conditions	2-8°C
Safety level	1
morphology	Small filamentous fungus. Colony formation is distinct on comprehensive PDA medium with rapid growth. Mycelium appears as white, cottony growth spreading to cover the entire Agar plate. Produces yellow spores in later stages, turning the medium surface yellowish-brown.
Sharing mode	Public welfare sharing

Description

1. Name: Trichoderma longibrachiatum

2. BNCC No.: 341613

3. Biosafety level: 4

2. Storage conditions:
Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1. Comprehensive PDA:20% potato juice 1L, glucose 20g ,KH2PO4 3g, MgSO4.7H2O 1.5g, thiamine trace, agar 15g,pH natural.

2. Atmosphere: aerobic

3. Temperature: 25 ℃

4. Notes:
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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