Bacillus subtilis|347725 |BNCC

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Bacillus pumilus

Subculture procedure	① Prepare 2 plates; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL sterile water into the freeze-dried vial, dissolve completely, and mix thoroughly; ④ Pipette 0.2mL bacterial suspension onto the Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all plates under specified conditions until colonies emerge.
Growth conditions	30°C; aerobic; 24h;
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 0.5–1 mm; circular, with neat edges; opaque, light-colored; raised center; smooth, shiny surface; moist texture; easily liftable; G– (red); bacillus; purity: pure
Sharing mode	Public welfare sharing

Description

1. Name : Grimontia hollisae

2, BNCC No.: 347725

3. Biosafety level:4

2. Storage conditions:
Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1, 2216E seawater agar: peptone 5.0g, yeast extract 1.0g, ferric citrate 0.1g, potassium bromide 0.08g,NaCl 19.45g,MgCl2 8.8g,Na2SO4 3.24g,CaCl2 1.8g,KCl 0.55g,NaHCO3 0.16g,NH4NO3 0.0016g,Na2HPO4 0.008g, strontium chloride 0.034g, boric acid 0.022g, sodium silicate 0.004g, sodium fluoride, pH 7.4-7.8.

2. Atmosphere: aerobic

3, Temperature: 30 ℃,24h

4. Notes:
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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